Alpha Innotech Red Manual
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How to Use the Alpha Innotech Red Imaging System for Gel Documentation
The Alpha Innotech Red Imaging System is a versatile and compact instrument that allows you to capture digital images of stained DNA and protein gels using a UV transilluminator and/or white light. The system has a built-in software and a touch screen interface that does not require a computer connection. You can save your images to a network, a USB flash drive or print them directly using a digital thermo printer. In this article, we will guide you through the steps of using the Alpha Innotech Red Imaging System for gel documentation.
Step 1: Turn on the system and open the software
To start using the system, you need to turn on the computer, the camera, the instrument and the printer. The computer login username is Biology and the password is biology. After logging in, open the software by clicking on the icon on the desktop. You will see the main window with several buttons on the left side.
Step 2: Select the illumination source and the filter
Depending on the type of stain you used for your gel, you need to select the appropriate illumination source and filter. For example, if you used ethidium bromide or a fluorescent dye that excites under UV light, you need to select the UV button in the software and choose the EtBr filter from the filter wheel. If you used a colorimetric stain such as Coomassie or silver stain, you need to select the white light button in the software and choose no filter from the filter wheel. The filter wheel is located on the front of the instrument and requires manual manipulation.
Step 3: Adjust the image settings
After selecting the illumination source and the filter, you need to adjust the image settings to obtain a clear and optimal image of your gel. You can use the rings on the lens to adjust the focus, zoom and exposure. The bottom ring controls the focus, the middle ring controls the zoom and the top ring controls the exposure. You can also use the AutoExpose button in the software to automatically set an optimal exposure time. You can preview your image on the touch screen before acquiring it.
Step 4: Acquire and save your image
When you are satisfied with your image settings, you can acquire your image by pressing the Acquire button in the software. A contrast adjustment and a tool box window will open, allowing you to further adjust your image brightness and contrast, as well as perform some basic analysis such as measuring band sizes and intensities. You can save your image by pressing the Save button in the tool box window. You can choose to save your image to a network through a LAN connection, a USB flash drive or print it directly by connecting a digital thermo printer.
Step 5: Clean up and turn off
After saving your image, you need to clean up your work area and turn off all components of the system. You need to wipe off any stain residue from the transilluminator with water and paper towels. Do not cut bands on the transilluminator or use any organic solvents to clean it. You also need to turn off the software, camera, instrument and printer. Do not store any images on
the computer hard drive.
References
Redâ Imaging System From Alpha Innotech Biocompare Product Review
AlphaInnotech Imaging systems 071813
OrioleâFluorescent Gel Stain Instruction Manual - Bio-Rad Laboratories a474f39169