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Graft-vs.-host disease (GVHD) is an immunological disorder that occurs after bone marrow transplantation, and is mediated by host cytotoxic T lymphocytes (CTLs) against donor MHC class I molecules expressed on host tissues. We have shown recently that a murine model of GVHD in which recipient mice are deficient in the class I-like MHC, H60, develop chronic active GVHD of the skin (uveitis and dermatitis). Here we report that, despite an intact donor bone marrow, H60-deficient mice with chronic GVHD, have progressive loss of CD8+ cells in the uvea, thymus, and peripheral lymphoid organs. This is in contrast to the progression of chronic GVHD in H60-sufficient mice, where loss of CD8+ cells is delayed and tends to occur by apoptosis rather than necrosis. These observations establish a role for CD8+ cells in chronic GVHD, and suggest that chronic GVHD in H60-deficient mice may be due to a direct killing of host CD8+ cells by donor CTLs.Parental smoking and the onset of self-injurious behavior in children with autism.
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